BIS102 Hilt F05 MT2 -no answer

BIS102 Hilt F05 MT2 -no answer - 1 of 3 BIS 102 Name

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Unformatted text preview: 1 of 3 BIS 102 Name ________________________________________ Fall, 2005 Last First K. Hilt Second Midterm Score (100): ___________ pH = pK a + log {[b]/[a]} (K a ) (K b ) = 1 x 10-14 pH = - log [H + ] F = (q 1 q 2 ) / r 2 K b = x 2 / (y-x) G = H - T S K a = x 2 /(y - x) pH = (pK a 1 + pK a 2 )/2 R-group pK a s: D (3.9), E (4.2); H (6.0); K (10.5), R (12.5); C (8.3); Y (10.1) 1. Imagine that you have purified protein Z. Lets assume that it is a typical (i.e. average) protein. (2 pts.) a) Protein Zs molecular weight is probably about __________________ (give units). (2 pts.) b) Protein Z probably has subunits. This means that protein Z has ________________ structure. (fill in the blank with one word). (10 pts.) c) There are thousands of protein Z molecules in solution, in a test tube. The solution is made up mostly of buffer and some mercaptoethanol. Why is the buffer pH critical to the biological activity of protein Z? Be detailed and specific in your answer (i.e. do not just say that the pH has to be of protein Z?...
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This note was uploaded on 10/07/2009 for the course BIS 52192 taught by Professor Enochbaldwin during the Fall '09 term at UC Davis.

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BIS102 Hilt F05 MT2 -no answer - 1 of 3 BIS 102 Name

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