BIS102 Hilt F06 MT2 -no answers

BIS102 Hilt F06 MT2 -no answers - 1 of 2 BIS 102 Fall, 2006...

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1 of 2 BIS 102 Name ________________________________________ Fall, 2006 Last First K. Hilt Second Midterm Score (100): ___________ 1. ( 25 pts.) Hemoglobin has three different histidine residues that play different roles in the reversible binding of oxygen. One of them is the distal histidine. What are the other two? What are their roles (if there is more than one role, then give them all)? Then rank these histidines in order of importance (i.e. #1 is the most important in the process of reversibly binding oxygen, etc.). Then explain why you ranked them that way. 2. ( 25 pts.) The Sanger method and the Edman method are both excellent ways of labeling and identifying the N-terminal amino acid in a peptide sequence. Let’s imagine that we are back in the 1970’s, doing these two methods by hand (i.e. the protein sequenator has not been invented yet). What are the similarities and differences between these two techniques? Give a detailed discussion that shows that you know the chemicals, procedures, and concepts necessary to carry out the analysis of the N-terminal residue using both methods. Which method, Sanger or Edman, is better? Why?
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BIS102 Hilt F06 MT2 -no answers - 1 of 2 BIS 102 Fall, 2006...

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