{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}

BIS102 Hilt SSII02 MT1 -no answers

# BIS102 Hilt SSII02 MT1 -no answers - 1 BIS 102 Summer 2002...

This preview shows pages 1–2. Sign up to view the full content.

1 BIS 102 Name ________________________________________ Summer, 2002 Last First K. Hilt First Midterm Score (133): ___________ Equations: pH = pK a + log {[b]/[a]} (K a ) (K b ) = 1 x 10 -14 pH = - log [H + ] F = (q 1 q 2 ) / ε r 2 1. ( 15 pts.) Imagine that the amino acids cysteine, lysine, and arginine are present in a weakly buffered solution. You are going to use paper electrophoresis to separate the amino acids. What pH will you make the solution, such that when you spot the mixture of amino acids in the middle of the piece of paper shown below (top view), one amino acid will remain at the original spot, one will migrate to the left, and one will migrate to the right? Give your reasoning , show all calculations, and identify each spot . The (+) and (-) electrode positions are shown. The pK a 's of the R-groups of cys, lys, and arg are 8.30, 10.5, and 12.5, respectively. Assume that the pK a 's of the α -COOH and α -NH 3 + groups are 2.10 and 9.60 for each of the three amino acids. (-) (+) pH = ? 2. ( 8 pts.) When the electrophoresis in question #1 above is done, you will take the paper strip shown above, dry it, and then do what to it to visualize the amino acid spots? If all three amino acids

This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

### Page1 / 3

BIS102 Hilt SSII02 MT1 -no answers - 1 BIS 102 Summer 2002...

This preview shows document pages 1 - 2. Sign up to view the full document.

View Full Document
Ask a homework question - tutors are online