BIS102 Hilt SSII02 MT1 -no answers

BIS102 Hilt SSII02 MT1 -no answers - 1 BIS 102 Summer, 2002...

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1 BIS 102 Name ________________________________________ Summer, 2002 Last First K. Hilt First Midterm Score (133): ___________ Equations: pH = pK a + log {[b]/[a]} (K a ) (K b ) = 1 x 10 -14 pH = - log [H + ] F = (q 1 q 2 ) / ε r 2 1. ( 15 pts.) Imagine that the amino acids cysteine, lysine, and arginine are present in a weakly buffered solution. You are going to use paper electrophoresis to separate the amino acids. What pH will you make the solution, such that when you spot the mixture of amino acids in the middle of the piece of paper shown below (top view), one amino acid will remain at the original spot, one will migrate to the left, and one will migrate to the right? Give your reasoning , show all calculations, and identify each spot . The (+) and (-) electrode positions are shown. The pK a 's of the R-groups of cys, lys, and arg are 8.30, 10.5, and 12.5, respectively. Assume that the pK a 's of the α -COOH and α -NH 3 + groups are 2.10 and 9.60 for each of the three amino acids. (-)
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This note was uploaded on 10/07/2009 for the course BIS 52192 taught by Professor Enochbaldwin during the Summer '09 term at UC Davis.

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BIS102 Hilt SSII02 MT1 -no answers - 1 BIS 102 Summer, 2002...

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