BIS102 Hilt W02 FINAL -no answers

BIS102 Hilt W02 FINAL -no answers - 1 Biological Sciences...

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1 Biological Sciences 102 Name ___________________________________________ Winter, 2002 Last, First K. Hilt Final Exam Instructions: You may use the back of these pages if you clearly indicate that you are doing so. Page Value Score 2 72 3 30 4 31 5 24 6 43 Total: (200) Compound pK a ’s Phosphate 2.12, 7.21, 12.32 Tris 8.21 Amino acid side chains asp 3.90 glu 4.20 his 6.00 tyr 10.10 lys 10.50 cys 8.30 arg 12.50 Amino acid α -carboxyl group 2.10 Amino acid α -amino group 9.60 Oligopeptide N-terminal amino group 7.40 Oligopeptide C-terminal carboxyl group 3.60 pH = -log [H + ] pH = -log a H+ K a = x 2 /(y - x) pH = (pK a 1 + pK a 2 )/2 pH = pK a + log {[base]/[acid]} (K a ) (K b ) = 1.00 x 10 -14 K b = x 2 / (y-x) G = H - T S F = (q 1 q 2 )/( ε r 2 ) a = γ c v o = {V max [S]} / {K m + [S]}
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2 BIS 102 Name ______________________________ 1. ( 6 pts.) There are certain requirements of a protein sample that must be met before that protein sample can be sequenced. Let's imagine that we are going to use trypsin to cut our protein sample. Identify three things that must be true about our protein sample before we can cut with trypsin: #1 ____________________________________________________________________ #2 ____________________________________________________________________ #3 ____________________________________________________________________ 2. ( 9 pts.) Why is protein sequencing important in biochemistry? Give three different reasons. #1 ____________________________________________________________________ #2 ____________________________________________________________________ #3 ____________________________________________________________________ 3. The hydrophobic effect was discussed at several points in our course. ( 5 pts.) a) What is the basis of the hydrophobic effect? ( 6 pts.) b) Identify two different reagents you could add to a buffer solution that would decrease the hydrophobic effect. #1 _____________________________ #2 _____________________________ 4. ( 6 pts.) An organism that needs to diminish the fluidity of its membranes is most likely to have more linolenic acid than oleic acid in its membranes. True or false? Explain, on a molecular basis, why this is true or
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BIS102 Hilt W02 FINAL -no answers - 1 Biological Sciences...

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