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BIS102 Hilt W02 MT2 -no answers

BIS102 Hilt W02 MT2 -no answers - 1of 2 Biological Sciences...

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1of 2 Biological Sciences 102 Name ____________________________________________ Winter, 2002 Last, First K. Hilt Second Midterm Score: ________________(100) 1. ( 10 pts.) A protein was completely digested with arg-C and an internal peptide (i.e. not derived from the N- or C-terminals) was purified. Determine its sequence from the following information: a) amino acid analysis of one mole of the peptide yields two moles of cysteine, one mole of arginine, one mole of methionine, and one mole of lysine; b) treatment of the original peptide with cyanogen bromide yields a tetrapeptide and free methionine; c) treatment of the original peptide with trypsin yields a single product; d) treatment of the original peptide with mercaptoethanol yields a single product; e) treatment with trypsin followed by treatment with mercaptoethanol yields two products. Sequence: _________________________________________(use the 3-letter code for the amino acid residues). 2. ( 5 pts.) Which level(s) of protein structure is(are) stabilized principally by the hydrophobic effect?
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