BIS102 Hilt W06 FINAL -no answers

BIS102 Hilt W06 FINAL -no answers - 1 of 3 Biological...

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Unformatted text preview: 1 of 3 Biological Sciences 102 Name ___________________________________________ Winter, 2006 MWF Last, First K. Hilt Final Exam Score (200): ______________ pH = pK a + log {[base]/[acid]} G = H - T S F = (q 1 q 2 )/( r 2 ) v o = {V max [S]} / {K m + [S]} PK a s of the side chain R-groups are: D (3.9), E (4.2), H (6.0), C (8.3), Y (10.1), K (10.5), and R (12.5). PK a s of amino acid -amino group (9.60) and -carboxyl group (2.10). PK a s of N-terminal oligopeptide - amino group (7.40) and C-terminal -carboxyl group (3.6). 1. (15 pts.) A student wants to purchase some trypsin to use in protein sequencing. Company A offers 200 I.U.s of the enzyme with a specific activity of 405 I.U./mg at a price of $200. Company B offers 150 I.U.s of the enzyme with a specific activity of 450 I.U./mg at the same price. Company C offers 405 I.U.s of the enzyme with a specific activity of 200 I.U./mg at the same price. Which company(s) should you buy from? a) Company A b) Company B c) Company C d) Either company A or C e) Either company A or B f) Either company B or C Now briefly explain your answer. Show any necessary calculations. 2. (25 pts.) The first workable fraction in enzyme purification is the ___________________________....
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This note was uploaded on 10/07/2009 for the course BIS 52192 taught by Professor Enochbaldwin during the Winter '09 term at UC Davis.

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BIS102 Hilt W06 FINAL -no answers - 1 of 3 Biological...

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