ch. 21 - Genomics, Bioinformatics and Proteomics Textbook...

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Genomics, Bioinformatics and Proteomics Textbook reading: Chapter 21 Objectives: To understand the steps involved in the Human Genome Project. To have a basic understanding of structural, functional and comparative genomics. To understand the development and significance of proteomics. Genomics - the study of genomes - structural genomics: development of sequence data, gene discovery, gene maps - functional genomics: expression and regulation of gene and function of their products - comparative genomics: identify functional and evolutionary relationships of genes Proteomics - the study of cellular proteins present in a cell - identification of all cellular proteins expressed under given set of conditions - identification of post-translational modifications to proteins - identification of protein-protein interactions within a cell - identification of the subcellular localization of proteins
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Human Genome Project Project goals to identify all the human genes (20,000 - 25,000) to identify all the human genes (20,000 - 25,000) to determine the sequences of the 3 billion base pairs in human genome to determine the sequences of the 3 billion base pairs in human genome to establish databases with the accumulated information to establish databases with the accumulated information improve tools for data analysis improve tools for data analysis transfer related technologies to the private sector transfer related technologies to the private sector address the legal, ethical and social issues that may arise address the legal, ethical and social issues that may arise http://www.ornl.gov/sci/techresources/Human_Genome/home.shtml Random facts The sequence would fill 200 500-page phone books If you recited the genome at a rate of 1 letter per second for 24hrs it would take 100 years.
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Method used by government sponsored consortium Clone by clone or map based. Methodical method based upon assembly of a physical map to aid in assembling sequences into overlapping aligned sequences of a individual chromosome. Different vectors were used including BAC, YAC and plasmids
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Shot-gun cloning strategy for cloning and sequencing entire genome Watson Mol Bio Gene 6th Ed.
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The Institute of Genome Research (TIGR) Celera Genomics Private-sector biotech company started by J. Craig Venter Sequencing strategy; 3 randomly sheared or enzyme digested DNA pools are cloned into shot-gun libraries, sequenced, and then computer-based assembling of overlaps into a contiguous sequence. Uses 3 different sized libraries: 2 kb - 6-fold genome coverage 20 kb - 3-fold genome coverage 200 kb - 1fold genome coverage
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The human genome contains 3 billion nucleotide bases (A, C, T, and G). • The average gene
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This note was uploaded on 10/07/2009 for the course BIOLOGY 2203 taught by Professor Anthwal during the Fall '08 term at Temple.

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ch. 21 - Genomics, Bioinformatics and Proteomics Textbook...

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