Cell 1 - 1. Which of the following can not happen to...

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µµµ µµµ µµµ µµµ µµµ µµµ µµµ µµµ Page -2- 1. Which of the following can not happen to visible light as it passes through cells? a. It induces fluorescence b. It is absorbed c. Its refractive index changes d. Its speed changes e. It is diffracted. 2. The phase-contrast microscope: a. Converts differences in relative brightness and darkness of a sample into differences in refractive index. b. Only the light diffracted by the specimen is captured by the objective lens, forming a cone of light. c. Uses the differences in the phase of light transmitted or reflected by a specimen to form distinct, contrasting images of the sample. d. Both a and b e. Both a and c 3. Which of the following does not contribute to the resolution attainable by a microscope? a. the wavelength of the light b. the magnification of the objective lens c. the refractive index of the material between the specimen and the lens d. the numerical aperture of the lens e. the magnification of the eyepiece 4. In a mixture of the five proteins listed below, which should elute third in Gel Exclusion Chromatography, assuming that you have chosen the correct gel for the column? a. Immunoglobulin G M(r)=145,000. b. Cytochrome c M(r)=13,000 c. Ribonuclease A M(r)=13,700. d. Serum Albumin M(r)=68,500 e. RNA Polymerase M(r)=450,000. 5. In a comparison of electrophoresis with and without SDS. The force that moves the samples in each case is: a. With SDS, it is the electric field, without SDS it is the electric field. b. With SDS, it is the electric field, without SDS it is the charge on the sample. c. With SDS, it is the charge on the sample, without SDS it is the electric field. d. With SDS, it is the charge on the sample, without SDS it is also the charge on the sample. e. None of the above. This is too confusing.
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Page -3- 6. What microscopy technique would be best to use to screen protein expression in zebrafish that were carrying the gene for GFP? a. A high resolution bright field microscope b. A phase contrast microscope c. A fluorescence microscope d. A transmission electron microscope e. A polarization microscope. 7. All of the following are properties of water except. a. High boiling point. b. High specific heat c. Expands when it freezes d. dissolves ionic compounds e. dissolves aliphatic compounds 8. If you were designing a protein and wanted to make it more hydrophobic, what two amino acids could you add to its sequence to get the best result?
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This note was uploaded on 10/07/2009 for the course BIOLOGY 3096 taught by Professor Sheffield during the Spring '09 term at Temple.

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Cell 1 - 1. Which of the following can not happen to...

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