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Cell 1 - 1 Which of the following can not happen to visible...

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°±²³´ µµµ µµµ µµµ µµµ µµµ µµµ µµµ µµµ µµµ ¶³· ¸ ¹ ° º »¼½¾ ¿·À»ÁÂà ¾Ä Page -2- 1. Which of the following can not happen to visible light as it passes through cells? a. It induces fluorescence b. It is absorbed c. Its refractive index changes d. Its speed changes e. It is diffracted. 2. The phase-contrast microscope: a . Converts differences in relative brightness and darkness of a sample into differences in refractive index. b . Only the light diffracted by the specimen is captured by the objective lens, forming a cone of light. c. Uses the differences in the phase of light transmitted or reflected by a specimen to form distinct, contrasting images of the sample. d. Both a and b e. Both a and c 3. Which of the following does not contribute to the resolution attainable by a microscope? 4. In a mixture of the five proteins listed below, which should elute third in Gel Exclusion Chromatography, assuming that you have chosen the correct gel for the column? 5. In a comparison of electrophoresis with and without SDS. The force that moves the samples in each case is:
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Page -3- 6. What microscopy technique would be best to use to screen protein expression in zebrafish that were carrying the gene for GFP? a. A high resolution bright field microscope b. A phase contrast microscope c. A fluorescence microscope d. A transmission electron microscope e. A polarization microscope.
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