DNA Sequencing - Learning Objectives Summarize the events of DNA replication 2 Discuss functions of major proteins required for DNA replication 3

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Learning Objectives 1. Summarize the events of DNA replication. 2. Discuss functions of major proteins required for DNA replication. 3. Discuss the dideoxy chain termination technique for DNA replication. 1. Compare/contrast manual DNA sequencing and automated sequencing. 2. Explain why the bottom of the sequencing gel is always the 5’ end of the molecule.
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DNA Replication The polarity of DNA is important structurally and functionally. 5’ refers to the 5’ carbon of deoxyribose to which the phosphate group is attached. 3’ refers to the 3’ carbon to which a hydroxyl group is attached.
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Extension from 3’ -OH DNA polymerase requires a free 3’ – OH to extend the nucleotide chain. Primase produces a short RNA that serves as a primer.
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Leading Strand The 3’ -> 5’ template strand produces a 5’ -> 3-’ replicated strand. Therefore the replicating 5’ -> 3’ strand is synthesized as one continuous piece, the leading strand.
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DNA polymerase cannot extend from a 5’ end. 5’-> 3’ template is
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This note was uploaded on 10/07/2009 for the course BIOL 3461 taught by Professor Mackinnon during the Fall '09 term at University of the Incarnate Word.

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DNA Sequencing - Learning Objectives Summarize the events of DNA replication 2 Discuss functions of major proteins required for DNA replication 3

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