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DNA Sequencing & BLAST Searches - DNA Sequencing BLAST...

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DNA Sequencing & BLAST, Protein Searches (based on EDVOTEK Kit #339 &340, Sequencing the Human Genome & DNA Informatics) Content & Process Skills: 1. Interpret an autorad film and an automated sequence printout to determine a DNA sequence. 2. Utilizes NCBI databases to conduct a BLAST search of aligned DNA sequences. 3. Utilize the NCBI “Science Primer” to acquire knowledge about the following: SNPs, ESTs, Microarrays Introduction: Bioinformatics is a new discipline that utilizes public and private databases of DNA sequences. The Human Genome Project was completed in 2002 and provided the complete nucleotide sequence of human DNA. Analyses of the sequences will allow for estimation of the number of genes in humans (originally thought to be about 80,000 but in 2005 the estimate is closer to 30,000). Genomes of many other organisms have also been completed. These sequence databases can be used to tentatively identify the structure and function of gene product and/or gene sequences.
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