Chapter five ua - CHAPTER FIVE Microscopic Examination Of Urine 1 Acknowledgements \u2022 \u2022 \u2022 \u2022 \u2022 \u2022 \u2022 Addisa Ababa University Jimma

Chapter five ua - CHAPTER FIVE Microscopic Examination...

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CHAPTER FIVE Microscopic Examination Of Urine 1
Acknowledgements Addisa Ababa University Jimma University Hawassa University Haramaya University University of Gondar American Society for Clinical Pathology Center for Disease Control and Prevention-Ethiopia 2
Chapter Objective At the end of this chapter the students will be able to describe Microscopic examination for urine sediment Normal and abnormal organized urine sediments with their diagnostic features. Formation and significance of casts Normal and abnormal crystals encounter in urine sediments Relationship between sediments, chemical, physical findings in urine Reporting of urinary sediments Quality control in urinalysis. 3
Chapter Outline Microscopic Examination Of Urine Procedure for microscopic examination Source of errors in the microscopic examination of urine Urinary Sediments Organized Urinary Sediments Parasite, fungus and bacteria in urine Non-Organized Urinary Sediments Urine Crystals Body cells, crystals, casts, yeasts, bacteria, sperm Methods of reporting formed elements Quality control in urinalysis. 4
Introduction In examining urinary sediment it is necessary to learn the distinguishing characteristics of elements which have clinical significance . If the urine sample is properly collected, the test is carefully performed, and the person performing the test is knowledgeable, skillful valuable information can be obtained from microscopic examination of urine specimen. Normally small amount of solid substances is found in the urine. But when their concentration become high, may indicate the existence of abnormal physiological function of our body. 5
Standardization Aspects of microscopic examination that should be standardizes: 1. Volume of urine analyzed 2. Length and force of centrifugal 3. Re-suspending volume and concentration of sediment 4. Volume and amount of sediment examined 5. Terminology and reporting format 6
Principles of method Centrifuge 10-12 ml urine specimen at 1500 – 2000 rpm for 3-5 minutes then discard the supernatant by quick inversion of the tube, resuspend and take the sediment by Pasteur pipette from the tube, transfer a drop into the clean and dry slide finaly examin on the microscope Repeated evaluation of urine sediment is frequently valuable in following the course and management of urinary tract disorders, because the appearance of cellular elements, and casts in the urine is a reflection of changes that take place in the kidney. 7
Reagents and equipment Assemble all necessary materials used for the collection, centrifugation and examination. centrifuge. Conical centrifuge tubes, or regular test tubes. Pasture pipette with rubber fit or automatic pipettes if possible.

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