opt rd 2 southern blotting

opt rd 2 southern blotting - Southern Blot Method

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Southern Blot Method http://www.bio.davidson.edu/courses/genomics/method/Southernblot. .. 1 of 3 1/24/07 10:34 AM Southern Blot Method This is a brief overview of how a Southern blot (more formally called an DNA blot) is performed and what type of data you can obtain form one. Figure 1. Southern blots allow investigators to determine the molecular weight of a restriction fragment and to measure relative amounts in different samples. Procedure 1) DNA (genomic or other source) is digested with a restriction enzyme and
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Southern Blot Method http://www.bio.davidson.edu/courses/genomics/method/Southernblot. .. 2 of 3 1/24/07 10:34 AM separated by gel electrophoresis, usually an agarose gel. Because there are so many different restriction fragments on the gel, it usually appears as a smear rather than discrete bands. The DNA is denature into single strands by incubation with NaOH. 2) The DNA is transfered to a membrane which is a sheet of special blotting paper. The DNA fragementsw retain the same pattern of separation they had
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This note was uploaded on 10/07/2009 for the course GEN 409 taught by Professor Linda during the Fall '09 term at Iowa State.

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opt rd 2 southern blotting - Southern Blot Method

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