prob set 8

prob set 8 - Problem Set 8 1) In a temperature sensitive...

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Problem Set 8 1) In a temperature sensitive genetic screen to identify mutations in genes that were necessary for DNA replication, two classes of mutations were isolated. Class I: When these cells were placed at the non-permissive temperature, radiolabeled nucleotides were no longer incorporated into DNA. WHY? Class II: When these cells were placed at the non-permissive temperature, they continued to incorporate radiolabeled nucleotides into newly synthesized DNA for a short time (~ 20 minutes). WHY? 2) (Multiple Choice) Eukaryotic replicons have the following properties. They: a) are much shorter than prokaryotic replicons because of the presence of termination sequences. b) are much longer than prokaryotic replicons because of the larger genome. c) Are usually bidirectional and able to fuse. d) All ‘fire’ at once to ensure that the entire genome is replicated during S-phase. 3) Several temperature-sensitive mutant strains of
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prob set 8 - Problem Set 8 1) In a temperature sensitive...

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