Lecture 03_09

Lecture 03_09 - INTRODUCTION TO CELL BIOLOGY AND METHODS...

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INTRODUCTION TO CELL BIOLOGY AND METHODS CHAPTER 1 LECTURE 3
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Lecture 3 Overview of Cellular Structure and Function Overview of Cellular Structure and Function (animal cell) + + + + _ _ _ (rough) smooth ER (=microtubule organizing center; MTOC) exo/endocytosis
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1. Cell Surfaces : The extracellular coat on the surface of the plasma membrane •The extracellular matrix (ECM): a complex network of structural fiber-like proteins and polysaccharides (e.g.,collogen); contributes to structural support, cell motility, wound healing. •Plants, fungi, and bacteria a cell wall (= ECM) covers the plasma membrane 1. Cell Surfaces : The extracellular coat on the surface of the plasma membrane •The extracellular matrix (ECM): a complex network of structural fiber-like proteins and polysaccharides (e.g.,collogen); contributes to structural support, cell motility, wound healing. •Plants, fungi, and bacteria a cell wall (= ECM) covers the plasma membrane Surface of sea urchin oocyte (Dr. Douglas Chandler) Surface of sea urchin oocyte (Dr. Douglas Chandler)
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Fibroblasts (F) embedded in a dense mesh of collagen (C) fibers that they produced. F F F C C C
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Plant Cell Wall Cellulose: structural polysaccharide consisting of long chains of glucose subunits linked by ß1-4 covalent bonds.
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2. Membranes: • The plasma membrane interacts with the extracellular environment and regulates transport of large and small molecules and ions into and out of the cell. • Cytoplasmic membranes are the intracellular membranes of organelles and vesicles . • Proteins associate with all cellular membranes providing various critical functions: transporters, enzymes, recognition sites, etc.
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3. The Nucleus:
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• Sequesters DNA from the rest of the cytoplasm by a double membrane: the nuclear envelope. •The inner membrane is supported by a network of strong protein filaments (intermediate filaments) called the nuclear lamina. •The outer membrane is continuous with the endoplasmic reticulum (ER), thus the perinuclear space is continuous with the lumen of the ER. • Like rough ER, the outer membrane is decorated with ribosomes. • A centrosome is associated with outer surface of nucleus. •Nuclear pore complex (NPC). Gated traffic through the NPC, occurs both ways.
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•NPCs are formed at sites where the inner and outer membranes of the nuclear envelope are joined.
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The nucleolus is the region of the nucleus where ribosomal RNA is transcribed. Typically eukaryotic cells contain one or two nucleoli. They are large in size and often easily seen with light microscopy euchromatin
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4. Mitochondria (tread-like granule):
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Lecture 03_09 - INTRODUCTION TO CELL BIOLOGY AND METHODS...

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