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bio note slides 1 - Light Life Lecture 1 notes Physical...

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Light & Life Lecture 1 notes
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Physical nature of light Functions: -Energy source sustains life (sun: mass E) -provides info about physical world ex) alga C. Reihnhardtii -photosynthetic w/ eyespot
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light is… portion of electromagnetic spectrum seen = ‘light’ (400nm to 700nm) -wavelength not in the spectrum= ‘radiation’ (UV, infrared) -properties: both particles and wave
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photons Light particles: contain certain amounts of energy (E inverse to λ) ex) blue= higher E, shorter λ red= lower E, higher λ
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Photon Photon can interact with matter by being: -reflected -transmitted - absorbed (used by organisms) molecules that absorbs light (ph) = pigment
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pigments Different pigment = absorbs different wavelength (ex. blue) -chlorophyll a (photosynthesis) -retinal (vision) -indigo (dye)
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Pigments absorb , how? Chemical structure: alternating C double bonds, ‘ conjugate system’ e delocalization ONE e of pigment can interact with ONE photon
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Chlorophyll absorption: e can ONLY be excited by 2 E levels (from ground): -level 2 (red λ photon) -level 3 (blue λ photon) - green λ (E) doesn’t match E levels of chlorophyll reflected
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Pigment colour is λ it cannot absorb chlorphyll is green (plants)
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‘Action spectrum’ = plot of the effectiveness of a biological process (ie photosynthesis) to absorb different λ ex) red/ blue λ are more effective than λ for driving photosynthesis
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Light as E source: (photosynthesis) e excited (by photon) has potential E used for work (in ETC to make ATP, NADH etc chemical E) = photosynthesis
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Light E in other processes: Light is used by halobacterium (prokaryote): -protein complex: ‘bacteriorhodopsin’ = light-dependent protein pump ( ATP formation)
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Light as a source of info: Organisms perceive the world in different ways (eye)
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