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BIPN140Lecture1 - Lecture 1 Biology 140 Reading Chapter 1...

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Lecture 1          September 25, 2008 Biology 140                     Reading: Chapter 1 Neurons and Glia Introduction Diversity and complexity Underlying simplicity: stereotyped signals Neurons Cell body Processes Dendrites Axons Synapses Diversity & its implications Input, integration and output Methods of study Methods for structural visualization: staining, dye injection, antibodies, RNA probes,  genetically encoded reporters (such as GFP) Methods for recording signals: electrical: intracellular & extracellular  voltage  recordings (resting potentials; action &  synaptic potentials) electrical: intracellular  current  recordings electrical: sharp microelectrodes, patch pipets,  extracellular electrodes optical: intrinsic signals (e.g. birefringence),  voltage-sensitive dyes,  calcium-sensitive dyes Intact nervous system: “in vivo” cells function normally; circuitry intact 1
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