Lect-5-SV - 10-08-09: Lecture 5 RECAP: Bioenergetics:...

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1 Free Energy ( Δ G) Course of rxn products reactants Δ G E a A + E A E B + E Energy of activation Transitional state A B reactant product Δ G < 0 RECAP: Bioenergetics: Reactions inside a living cell Energy of activation With enzyme δ G < 0 – so the reactions looks spontaneous but actually requires and enzyme! 10-08-09: Lecture 5
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2 RECAP: Bioenergetics: Enzymes are substrate specific (SPECIFICITY) There can be > 1 in a reactions. Substrate is acted on by the enzyme. Basic Properties of Enzyme Active Site: pocket on enzyme where substrate can bind Specificity: compatible fit between enzyme and substrate (remember R-groups - chemical toolbox) Induced Fit: substrate binding induces 3D structural change of Enzyme Chemistry takes place with reactants (transitional states) 10-08-09: Lecture 5
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3 Enzyme Kinetics: Reaction rates with and without and enzyme Reaction rate (V) [Substrate conc.] A B reactant product Δ G < 0 Saturation Curve: substrate is in vast abundance to study enzyme kinetics Saturation Curve [ex. O2 partial pressure] ex. Myoglobin – monomeric hemoprotein 10-08-09: Lecture 5
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4 Enzyme Kinetics: Reaction rates with enzyme Saturation Curve [Substrate conc.] Reaction rate (V) 10-08-09: Lecture 5
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5 Enzyme Kinetics: Reaction rates with enzyme Saturation Curve Reaction rate (V) [Substrate conc.] Since the substrate concentration at V max cannot be measured exactly, enzymes must be characterized by the substrate concentration at which the rate of reaction is half its maximum. Michaelis-Menten constant (K M ) 10-08-09: Lecture 5
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6 Enzyme Kinetics: Reaction rates with enzyme Enzyme Regulation affects the reaction RATE (V) Saturation curves helps determine type of regulation!!
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Lect-5-SV - 10-08-09: Lecture 5 RECAP: Bioenergetics:...

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