enzyme 2 data

enzyme 2 data - Figure 5: Peroxide Concentration and...

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Unformatted text preview: Figure 5: Peroxide Concentration and Reaction Rate at 24.5 Degrees C 0.03 0.02 0.02 Reaction Rate (Products formed/second) 0.01 0.01 0 0 0.02 0.04 0.06 0.08 0.1 0.12 0.14 Peroxidase Concentration (M) Figure 4: Peroxidase Reaction at 4 Degrees C and Room Temperature (24.5 degrees C) 0.03 0.03 0.02 Reaction Rate (products formed/sec) 0.02 Column I Column J 0.01 0.01 0 0 0.02 0.04 0.06 0.08 0.1 0.12 0.14 Peroxide Concentration (M) Figure 5: Peroxidase Reaction at 45 degrees C, 55 degrees C, and 65 Degrees C 0.08 0.07 0.06 0.05 Reaction Rate (Products formed/sec) 0.04 Column K Column L Column M 0.03 0.02 0.01 0 0 0.02 0.04 0.06 0.08 0.1 0.12 0.14 Peroxide Concentration (M) TEMPERATURE Hydrogen peroxide conc. (M) 0.02 0.03 0.02 0.01 0.02 0.01 0 0 Initial velocity (amount of product formed per second) 0.02 0.02 0.02 0.02 0.01 0.01 0 0 0.07 0.07 0.05 0.04 0.02 0.03 0 0 0.03 0.04 0.04 0.03 0.01 0.01 0 0 0.08 0.05 0.01 0.03 0.01 0.01 0 90 0 120 0 0 0 0 0 0 0 0 0.13 0.03 0.01 0 4 oC 0.02 0.01 0.02 0 7.81 24.5 oC 0.02 0.02 0.01 0 45 oC 0.07 0.04 0.02 0 Average Average Average Average Figure 14: Lineweaver-Burk Plot ofof Peroxidase Reaction atat 65 Degrees C Figure 13: Lineweaver-Burk Plot the Peroxidase Reaction 55 Degrees C Hydroxylamine (10%) Hydrogen peroxide concentratio Conc. of n (M) inhibitor (%) 0.13 f(x) 80 f(x) = = 0.65x + 22.16 0.43x + 22.41 100 Initial velocity (amount of product formed per second) 70 0 0.01 1 0.03 2 0.01 60 80 50 60 1/[Reaction Rate] 1/(Reaction rate) 40 0.01 0.01 0 0 0 0 0 0 30 40 20 20 10 00 00 7.81 31.25 125 500 Figure 12: Lineweaver Burk P Average 0.03 Average 0.01 Average 0.03 0.02 0.01 0.02 0.01 0.03 0.03 0.02 0.01 0.01 0.01 0 0 60 14.29 22.47 48.78 666.67 = 0.29x + 12.63 50 f(x) 20 20 40 40 60 60 1/[H2O2] 1/[H2O2] 80 80 40 100 100 30 120 120 140 1/(Reaction rate) 20 20 20 10 00 00 50 f(x) = 0.29x + 12.63 0 Average 0.01 0 0 0 0 0 0 0 20 20 40 40 60 60 1/[H2O2] 1/[H2O2] 80 80 40 100 100 30 120 120 140 1/(Reaction rate) 20 0.03 Figure 7: Peroxide Concentratio 10 0 0.03 0 20 0.02 Figure 6: Peroxide Concentration and Reaction Rate at 45 Degrees C 0.08 0.07 Reaction Rate (Products formed/sec) 0.06 0.02 0.01 0.05 0.04 0.03 0.02 0.01 0 0 0.02 0.04 0.06 0.08 0.1 0.12 0.14 Peroxide Concentration (M) 0.01 Reaction Rate (Products Formed/second) 0 0 0.01 0 0 0.02 0.04 0.06 0.08 0.1 0.12 0.14 Peroxide Concentration (M) Figure 6: Peroxide Concentration and Reaction Rate at 75 Degrees C 1 0.9 0.8 0.7 0.6 Reaction Rate (Products formed/sec) 0.5 0.4 0.3 0.2 Reaction Rate (Products Formed/second) 0.08 Figure 7: Peroxide Concentration and Reaction Rate at 55 degre 0.07 0.06 0.05 0.04 0.03 0.02 0.01 0.04 0.04 0.03 0.03 0.02 0 Reaction Rate (products formed/second) 0.1 0 0 0 0.02 0.04 0.06 0.08 0.02 0.1 0.02 0.04 0.06 0. 0.12 0.14 Peroxide Concentrat 0.01 Peroxide Concentration (M) 0.01 Figure 6: Peroxide Concentration and Reac 0 0 0.02 0.04 0.06 0.08 Peroxide Concentration (M) 0.01 0.01 0 0 0.02 0.04 0.06 0.08 Peroxide Concentration (M) Figure 12: Lineweaver-Burk Plot of Peroxidase Reaction at 45 Degrees C 60 f(x) = 1.12x + 13.7 50 40 1/(Reaction Rate0 30 20 10 0 -100 -80 -60 -40 1/[H2O2] -20 0 20 40 10 0 -100 -80 -60 -40 1/[H2O2] -20 0 20 40 55 oC 0.03 0.03 0.01 0 65 oC 0.07 0.02 0.01 0 75 oC 0 0 0 0 tat 65 Degrees C 55 Degrees C 41.67 71.43 66.67 333.33 14.29 22.47 48.78 666.67 7.81 31.25 125 500 15.38 57.14 100 1000 2: Lineweaver Burk Plot of Peroxidase Reaction at 45 Degrees C 60 7.81 31.25 125 500 29.41 31.25 76.92 50 f(x) = 0.29x + 12.63 40 00 00 30 120 120 140 ) 20 50 f(x) = 0.29x + 12.63 40 00 00 30 120 120 140 ) 20 eroxide Concentration and Reaction Rate at 4 Degrees C 10 0.03 0 0 0.03 20 0.02 40 60 1/[H2O2] 80 100 120 140 ts formed/sec) 0.02 0.01 0.01 0 0 0.02 0.04 0.06 0.08 0.1 0.12 0.14 Peroxide Concentration (M) ction Rate at 55 degrees C 04 0.06 0.08 0.1 0.12 0.14 Peroxide Concentration (M) Concentration and Reaction Rate at 45 Degrees C 4 0.06 0.08 0.1 0.12 0.14 eroxide Concentration (M) 4 0.06 0.08 0.1 0.12 0.14 eroxide Concentration (M) ...
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This note was uploaded on 10/11/2009 for the course BIOL 205 taught by Professor Chernin during the Fall '09 term at Bucknell.

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