enzyme lab data

enzyme lab data - Part 2 Peroxidase conc. (relative units)...

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Unformatted text preview: Part 2 Peroxidase conc. (relative units) Initial velocity (amount of product formed per second) Reaction Rate (Product formed/sec) 2 1 0.5 0.25 0.050 0.033 0.024 0.013 Figure 2: H 0.060 0.050 0.040 7.81 15.63 31.25 62.5 125 250 500 1000 1/(Reaction Rate) Initial velocity (amount of product formed per second) Part 3 Hydrogen peroxide Figure 3: Lineweaver-Burk conc. (M) 140.000 0.13 0.054 f(x) = 0.41x + 16.91 0.06 0.047 0.03 0.033 120.000 0.02 0.023 0.01 0.014 0 0.009 100.000 0 0.002 0 0.002 80.000 60.000 18.560 . 21.368 30.000 40.000 43.706 71.869 116.667 20.000 445.860 636.364 0.000 0 50 Plot of Peroxide Concen 0.030 0.020 0.010 0.000 0 100 150 1/[H2O2] Figure 2: Hydrogen Peroixde Concentration and Reaction Rate Reaction Rate (Product formed/sec) 0.060 0.050 0.040 lot of Peroxide Concentration and Reaction Rate 0.030 0.020 0.010 0.000 0 0.02 0.04 0.06 0.08 0.1 0.12 0.14 Hydrogen Peroxide Concentration (M) Initial Velocity of Reaction (products/sec) Figure 1: Peroxidase Concentration and Reaction Rate 0.060 0.050 0.040 0.030 0.020 0.010 0.000 0 0.5 1 1.5 2 2.5 Peroxidase Concentration (relative units) f(x) = 0.02x + 0.01 200 250 300 100 150 1/[H2O2] Figure 3: Lineweaver-Burk Plot of Hydrogen Pero 700.000 600.000 500.000 400.000 300.000 200.000 100.000 0.000 0 200 400 Inverse of Initial Velocity (seconds/reactants formed) 60 Inverse of Hydrogen peroxide Burk Plot of Hydrogen Peroxide Concentration vs. Reaction Rate 200 400 600 800 1000 1200 verse of Hydrogen peroxide Concentration (1/[M]) ...
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This note was uploaded on 10/11/2009 for the course BIOL 205 taught by Professor Chernin during the Fall '09 term at Bucknell.

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