peroxidase lab design

peroxidase lab design - Hannah Kotler How Do Different...

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Hannah Kotler How Do Different Temperatures Affect the Peroxidase Reaction? Hypothesis: The reaction rate will increase as temperature increases, until it reaches a certain optimal temperature. At this point the protein will begin to denature and the enzyme will cease to function. The independent variable is temperature at which the reaction proceeds. The dependent variable is reaction rate, as measured by absorption. Controlled Variables: peroxidase concentration, hydrogen peroxide concentration, pH, source Positive Control: Enzyme at room temperature (to be measured at time of experiment, but ~20 degrees C) Negative Control: Blank Procedure Prepare 14 test tubes according to Table 1 below. Table 1 Tube Guiacol (mL) Turnip Extract (mL) .064 M Hydrogen Peroxide (mL) Distilled Water 1,3,5,7,9,11,13 .1 1 8.9 2,4,6,8,10,12,1 4 .1 1 8.7 Leave tubes 1 and 2 at room temperature (to be measured on day of experiment). Place tubes 3 and 4 in an ice bath to get to 0 degrees Celsius. Place tubes 5 and 6
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peroxidase lab design - Hannah Kotler How Do Different...

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