Lab Directions Cellular Respiration

Lab Directions Cellular Respiration - The Johns Hopkins...

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The Johns Hopkins University General Biology Lab I Page 1 of 4 Cellular Respiration in Peas Goal: To detect cellular respiration in germinating peas. Overview: Cellular respiration is the disassembly of glucose to yield ATP. ATP is used by the cell to fuel many of its functions. In other words, the energy locked in glucose is liberated through cellular respiration. Cellular respiration requires the input of oxygen gas along with glucose. At the end of the process, water and another gas, carbon dioxide, are produced. You will use this knowledge to detect cellular respiration in germinating (just-sprouted) peas. These peas are growing rapidly and need lots of energy. The peas will be placed in a closed chamber. As they undergo cellular respiration, they will slowly use up the oxygen in the chamber. At the same time, they will give off carbon dioxide. However, this carbon dioxide will be immediately converted into a solid form by the KOH present in the chamber (see below). So, the net amount of gas in the chamber will slowly decrease. You'll use a dye indicator to witness the resulting loss of pressure in the chamber. There will be three closed chambers, or “respirometers” (meters that measure respiration). The first will have untreated, germinating peas. The second will have germinating peas that have been frozen and then thawed. This will simulate what happens when sprouting peas are subjected to a late spring frost. The third chamber will not have any peas in it. This tube is called a “thermobar”, and will be used to correct for changes in pressure in the room due to the temperature and weather changes. Notes: 1. You will work with a partner in this lab. 2. For your own safety, be very careful with the needles! Follow good needle disposal etiquette, as well: a. When you first unwrap the needle, save the plastic cap. b.
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This note was uploaded on 10/11/2009 for the course BIOLOGY 020.153 taught by Professor Pearlman during the Fall '09 term at Johns Hopkins.

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Lab Directions Cellular Respiration - The Johns Hopkins...

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