Lab Directions PCR - The Johns Hopkins University General...

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The Johns Hopkins University General Biology Lab I Ecosystems of the Homewood Campus: Part IV PCR Amplification of the 16S Ribosomal DNA Gene Purpose : To amplify the piece of DNA you will use to identify your unknown bacteria. You will accomplish this using PCR (polymerase chain reaction). For more background information on PCR, see the textbook and the DNA Learning Center’s PCR animation (a link to this website is on the course website). Notes : 1. Work with your partner. Together, set up one polymerase chain reaction (PCR) from your joint bacterial sample. 2. Maximize your PCR success by minimizing your contamination! The primers in your reaction may stick to the wrong piece of DNA if you give it enough DNA to choose from. Minimize the amount of contaminating DNA in your sample by… Wearing gloves Wiping down your bench with disinfectant before you begin Using aerosol-barrier tips for the micropipettors (Each time someone uses a micropipettor, there is a chance that DNA from their sample could aerosolize (spray up) and stick to the barrel. To keep this DNA from raining down on your sample, use tips with a small filter in the top.) 3.
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This note was uploaded on 10/11/2009 for the course BIOLOGY 020.153 taught by Professor Pearlman during the Fall '09 term at Johns Hopkins.

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Lab Directions PCR - The Johns Hopkins University General...

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