Lab Directions PCR - The Johns Hopkins University General...

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
The Johns Hopkins University General Biology Lab I Ecosystems of the Homewood Campus: Part IV PCR Amplification of the 16S Ribosomal DNA Gene Purpose : To amplify the piece of DNA you will use to identify your unknown bacteria. You will accomplish this using PCR (polymerase chain reaction). For more background information on PCR, see the textbook and the DNA Learning Center’s PCR animation (a link to this website is on the course website). Notes : 1. Work with your partner. Together, set up one polymerase chain reaction (PCR) from your joint bacterial sample. 2. Maximize your PCR success by minimizing your contamination! The primers in your reaction may stick to the wrong piece of DNA if you give it enough DNA to choose from. Minimize the amount of contaminating DNA in your sample by… Wearing gloves Wiping down your bench with disinfectant before you begin Using aerosol-barrier tips for the micropipettors (Each time someone uses a micropipettor, there is a chance that DNA from their sample could aerosolize (spray up) and stick to the barrel. To keep this DNA from raining down on your sample, use tips with a small filter in the top.) 3.
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 3

Lab Directions PCR - The Johns Hopkins University General...

This preview shows document pages 1 - 2. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online