{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}

Bis 104 Lecture 2

Bis 104 Lecture 2 - One globulin to multiple antigen to...

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
104 Lecture 2 Electron microscopy - greater detail on surface (3D), alot greater than light microscopy unaided eye can see .2 mm SEM – see surface outside PEM – see structure inside, can be stained Detecting proteins i. A typical antibody molecule(immunoglobulin, lg Human have 40,000ish gene Why can mammal make billions of different antibodies. NOT taught in bis104 Raising antibodies: Immunized host animal with antigen. Multiple injection to get stronger response thus creating stronger antibodies.
Background image of page 1
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: One globulin to multiple antigen to form a huge aggregation. Separation proteins Fractionation of cell contents by differential centrifugation Proteins can be separate by charge size and affinity, can also use column chromatography Q: When yo9u use a gel filtration column to purify proteins according to their sizes, proteins to be eluted first are. Larger ones coming out of column first Protein can be separate via SDS polyAcrylamide-Gel Electrophoresis...
View Full Document

{[ snackBarMessage ]}

Ask a homework question - tutors are online