Bis 104 Lecture 4

Bis 104 Lecture 4 - B is 104 Lectu re 4 Q:The stereocilia...

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Bis 104 Lecture 4 Q:The stereocilia progecting from the inner…. A: 4 b. how do we know a protein forms transmembrane domain? i. predicting transmembrane domains by hydropathy plots: how many passes through the membrane? Most common membrane-spanning protein structure is a alpha helix ii. Determing the topology of proteins inside the membrane: SDS-PAGE analysis of trypsin-digested protein samples -when trypsin digest protein on the membrane, if protein is exposed on the EM or IM, the more exposed will travel faster on the SDS-PAGE gel. *Membrane proteins are mobile as well. [they will diffuse evenly when two cell with different proteins fused together, but only at 37 degree Celsius after 40 minutes. c. But cells have developed sophisticated mechanisms to limit diffusions. Membrane proteins are often [there are sophisticated system to limit diffusion of protein throughout cell] such as…tight junction -protein form aggregation(sticks together), it limits how they can separate. -associate protein with other innercellular protein in the cell, so the selected protein
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Bis 104 Lecture 4 - B is 104 Lectu re 4 Q:The stereocilia...

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