bis_104_sample_exam_key_07 - BIS 104 NAME _ (last) (first)...

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BIS 104 NAME _________________________________________ (last) (first) MIDTERM EXAMINATION Consider the following in vitro experimental protocol: A pure population of undifferentiated endothelial cells (PECAM-1 positive; EphB2 negative; EphB4 negative) are was isolated from mouse embryo limb skin. These cells are from transgenic mice that have the gene coding for Green Fluorescent Protein (GFP) linked directly to the gene that codes for EphB2, a cell surface protein found only on differentiated arterial cells. These undifferentiated endothelial cells were co-cultured with a pure population of mouse Schwann cells for 2 days. After 2 days of co-culture you perform a series of experiments to test various aspects of a two part hypothesis, given below: (a) Schwann cells are capable of inducing endothelial cells to differentiate into arterial cells. (b) The Schwann cells accomplish this induction by synthesizing and secreting the growth factor protein, VEGF, which then acts through a receptor protein on the surface of the undifferentiated endothelial cells. Before beginning your experiments you wish to see exactly what your cell populations look like in their living state and to verify that they are normal and healthy. What is the most direct microscopic technique you could use to effectively assess the appearance of your live cells? Phase contrast microscopy or differential interference microscopy of live cells (no stains, no fixatives). Using this same microscopic technique, could you tell if your cell cultures are contaminated with bacteria such as Staphylococcus aureus , which have a spherical diameter of approx 0.8 um? Yes, since under optimal conditions your scope should be able to resolve objects as small as 0.2 um in diameter .
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This note was uploaded on 10/12/2009 for the course BIS BIS 104 taught by Professor Privalsky during the Fall '09 term at UC Davis.

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bis_104_sample_exam_key_07 - BIS 104 NAME _ (last) (first)...

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