Ebola Virus Research

S possible vaccine for ebola virus infection in

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Unformatted text preview: ylactic agent. S Possible vaccine for Ebola virus infection in primates. N The combination of naked DNA vaccine capable of encoding Ebola proteins followed by a booster vaccination with a recombinant adenoviral vector expressing Ebola GP(Z) L Cynomolgus macaques were injected with 3 doses of the DNA vaccine, 1 dose every 4 weeks. L 12 weeks later, the macaques were vaccinated with the recombinant adenoviral vector. L After another 12 weeks, unvaccinated macaques and vaccinated macaques were injected with a lethal dose of Ebola virus L All unvaccinated macaques died, while none of the vaccinated macaques died. L Result: Primates can be vaccinated against Ebola and can develop both a cellmediated response (thought to be a result of the DNA vaccine) and a humoral antibody response (thought to result from the recombinant adenoviral vaccine). N Using vaccine strategies that was successful in mice and guinea pigs L RNA replicon particles from an attenuated strain of Venezuelan equine encephalitis virus that expressed Ebola virus glycoprotein and nucleoprotein9 L Recombinant vaccina virus that expressed Ebola glycoprotein9 L VP24, VP30, VP35, and VP40 proteins11 L Liposomes containing lipid A L Inactivated Ebola virus L Selected EBOV genes have been cloned into plasmid vectors and administered by intramuscular injection or coated onto gold particles that were bombarded into the skin using a gene gun9 L Concentrated, inactivated whole-virion Ebola preparation L Result: Adult mice were protected against the Ebola virus by inducing measurable antibody responses detected by ELISA and induced cytotoxic T-cell immunity. It did not protect Cynomolgus macaques (M fascicularis) or rhesus macaques (Macaca mulatta). PREVENTION The prevention of Ebola HF in Africa presents many challenges.30 Because the identity and location of the natural reservoir of Ebola virus are unknown, there are few established primary prevention measures If cases of the disease do appear, current social and economic conditions often favor the spread of an epidemic within health-care facilities. The aim of all of these techniques is to avoid any person’s contact with the blood or secretions of any patient and to prevent further nosocomial transmission by using locally available materials and few financial resources Health-care providers must be able to Recognize a case of Ebola HF should one appear. Have the capability to perform diagnostic tests Be ready to employ practical viral hemorrhagic fever isolation precautions, or barrier nursing techniques27 Wearing of protective clothing, such as masks, gloves, gowns, and goggles Use of infection-control measures18 Complete equipment sterilization Isolation of Ebola HF patients from contact with unprotec...
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This note was uploaded on 10/13/2009 for the course YIPH ln;ljhdfi taught by Professor Wanger during the Spring '09 term at Heriot-Watt.

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