Class lec ol 11-12#2 DNA rep

Class lec ol 11-12#2 DNA rep - FLOW OF GENETIC...

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Unformatted text preview: FLOW OF GENETIC INFORMATION* (Please review these. If you have questions, see me) Terms to learn: Strain / clone Genome – all the DNA present in a cell or virus Haploid Diploid Genotype – specific set of genes it posseses Phenotype – observable characteristics DNA as genetic material Griffith’s experiment: Figure 11.1 Transformation of avirulent (rough) Streptococcus pneumoniae to the virulent (smooth) form • S pneumococci (virulent) that was heat killed and injected into mice did not kill the mice • However, heat killed plus live R pneumococco (nonvirulent) injected lead to death of mouse • Showed transformation of the virus Avery et al’s expt.: Figure 11.2 DNA as transforming agent • Showed that DNA must be present for transformation bc the DNA of the dead S cells are taken up by the live R cells • Only if treated with Dnase would there be no transformation 1 Hershey and Chase’s expt.: Figure 11.3 Labeling phage T2 with 32 P or 35 S. DNA as genetic material in T2 • used bacteriophage T2 infection as model • Demonstrated that DNA, not protein carried genetic material • Protein labeled with S35 not found in subsequent phages • DNA labeled with P32 was found in subsequent phages produced inside the cell 2 Watson and Crick: Figure 11.6, DNA double helix • Sugar-phosphate backbone (phospodiester bonds) spiral the outside of the double helix with the base pairs embedded inside • 3 hydrogen bonds between guanine and cytosine, 2 between adenine and thymine Flow of genetic information: • from one generation to the next – DNA stores genetic information – Information is duplicated by replication and is passed on to next generation • Process called gene expression • DNA divided into genes – transcription yields a ribonucleic acid (RNA) copy of specific genes – translation uses information in messenger RNA (mRNA) to synthesize a polypeptide • Also involves activities of transfer RNA (tRNA) and ribosomal RNA (rRNA) 3...
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This note was uploaded on 10/18/2009 for the course FALL 09 taught by Professor Saxena during the Fall '09 term at University of Texas.

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Class lec ol 11-12#2 DNA rep - FLOW OF GENETIC...

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