Exam_I-Form_A - Name: BCH 461 - Exam I September 19, 2008...

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Name: Student #: BCH 461 - Exam I September 19, 2008 This exam consists of 31 questions. Total points = 100. All questions except #31 are worth 3.3 points. Answers should be given on the bubble sheet. Choose only the one best answer. The use of calculators, books, or notes is NOT allowed on this exam. The exam begins at 9:10 A.M. and ends at 10:00 A.M. sharp! The time needed to fill in the scoring sheet is included. Nothing may be written after 10:00 A.M. This is FORM A. Mark this in the “form” field on your bubble sheet. __________________________________________________________________________ 1. Imagine that you are purifying from yeast a naturally occurring mitochondrial enzyme involved in heme biosynthesis. The most appropriate first technique to use in the purification of this enzyme from the cell is: (a) differential centrifugation (b) size exclusion chromatography (c) affinity chromatography (d) isoelectric focusing (e) salting out 2. Given what you know about hemoglobin and myoglobin, what would be the best technique for separating these two proteins from each other? Assume that you have a protein solution that contains both proteins. (a) differential centrifugation (b) size exclusion chromatography (c) affinity chromatography (d) isoelectric focusing (e) salting out 3. What is the approximate pI of the heptapeptide Val-Glu-Trp-Lys-Ser-His-Ile? (a) 4 (b) 8 (c) 11 (d) 7 (e) 6
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4. Urease has a pI of approximately 5.0. The best way to purify urease using ion exchange chromatography would be to load the enzyme onto a(n) _______________. (a) cation exchange column at pH 3 and slowly lower the pH to elute the protein (b) anion exchange column at pH 7 and slowly lower the pH to elute the protein (c) cation exchange column at pH 7 and slowly lower the pH to elute the protein (d) anion exchange column at pH 3 and slowly raise the pH to elute the protein (e) cation exchange column at pH 7 and slowly raise the pH to elute the protein 5. This amino acid contains an –R group that is a hydrogen bond acceptor at pH 8. (a) Lys (b) Phe (c) Thr (d) Gly (e) Ile 6. This amino acid contains an –R group that is typically found on the interior of the protein. (a) Lys (b) Arg (c) Glu (d) Asn (e) Leu 7. Imagine adding a concentrated NaOH solution to 175 mL of a 0.5 M solution of acetate with an initial pH of 3. In the first step you add enough NaOH to raise the pH from 3 to 4. In a second step you add additional base to this same solution to raise further the pH from 4 to 5. In which step did you add more moles of base? (The p K a of acetic acid is 4.76.) (a) Second step raising the pH from 4 to 5. (b)
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This note was uploaded on 10/18/2009 for the course BMB 461 taught by Professor Stoltzfus during the Spring '07 term at Michigan State University.

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Exam_I-Form_A - Name: BCH 461 - Exam I September 19, 2008...

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