Lect26 - Microbial genetics and biotechnology Learning...

Info iconThis preview shows pages 1–7. Sign up to view the full content.

View Full Document Right Arrow Icon
Microbial genetics and biotechnology
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Learning Goals Students will understand the difference between a selection and screen and when to use them Students will understand specific examples of using microbial genetics to solve problems and generate products in biotechnology Students will be introduced to the ethical dilemmas that biotechnology presents and the decisions society must make about biotechnology
Background image of page 2
Genetics as a thought process Start with a system ( cell ) Remove a component of the system ( mutation ) Observe the resulting perturbation of the system ( growth phenotype ) Deduce role of the relevant component in the functional system.
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Click to edit Master subtitle style Mutations help us understand phenotypes, and phenotypes help us understand mutations Wild-type Mutant protein Organism with mutant phenotype Classical genetics protein growth Mutant DNA Use the mutant to understand the wild-type Manipulate the wild-type to accomplish a task
Background image of page 4
Click to edit Master subtitle style Wild-type + + Mutant - + Consider growth of two strains: minimal + histidine What does the phenotype tell you about the mutation ?
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Screens and Selections 1. Selection : Define a condition where ONLY the cells with the phenotype of interest grow.
Background image of page 6
Image of page 7
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 10/18/2009 for the course MICROBIO 303 taught by Professor Kaspar/escalnte/downs during the Spring '09 term at Wisconsin.

Page1 / 25

Lect26 - Microbial genetics and biotechnology Learning...

This preview shows document pages 1 - 7. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online