Unit III notes - Unit III: Microbes Adapt, Microbial...

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Microbes Adapt, Microbial Genetics I. Lecture 3/24 A. Learning goals 1. 2. Understand boil. Flow of info 3. RNA polymerase = responsible for trxn DNA RNA 4. All genes encode RNA products 5. Expol. basic similarities & difs in chrom structure & DNA replication among bact/arch/euk 6. Understand base paired double helix & explain how it affects cell replication B. Central dogma 1. DNA RNA protein (or funct. unit) a. Replication b. Transcription c. Translation 2. Nucleic acid str. a. Bases : A, G, C, T, U b. Sugars: ribose, deoxyribose c. Nucleoside – base + sugar d. Nucleotide – base + sugar + phosphate e. Purines – adenine, guanine f. Pyrimidines – thymine, cytosine, uracil 3. chemistry generated differences a. auto calytic cleavage > RNA (2’ –OH) b. DNA = wider/shallower major groove & is less rigid (easier to store c. Thymine ? > because cytosine can easily degrade to uracil 4. Function dictates structure a. DNA = chemically stable, flexibility
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Unit III notes - Unit III: Microbes Adapt, Microbial...

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