3-2-09_gene_expression

3-2-09_gene_expression - Zoology 470 2009 Course handouts...

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Zoology 470 – 2009 – Course handouts Page 44 “Central Dogmatics” and Developmental Biology from The Way Life Works Campbell et al, 6e, Fig. 17.2b Campbell et al, 6e, Fig. 19.7
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Zoology 470 – 2009 – Course handouts Page 45 Campbell et al, 6e, Fig. 19.8 Campbell et al, 6e, Fig. 17.7 Gilbert 6e, Fig. 5.1 Becker et al., 4e, Fig. 21.10 Campbell et al, 6e, Fig. 19.9
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Zoology 470 – 2009 – Course handouts Page 46 Transcriptional Regulators Campbell et al, 6e, Fig. 17.9 Campbell et al, 6e, Fig. 17.8 Becker et al, 4e, Fig. 21.24 Gilbert 5e, Fig. 9.9 Gilbert 5e, Fig. 9.10a
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Zoology 470 – 2009 – Course handouts Page 47 adapted from Alberts et al, 3e, Fig. 7.39 Reporter Constructs Big idea : Fuse the regulatory DNA associated with a gene to a “reporter”. The reporter is transcribed wherever the normal gene would be transcribed, and can be detected by a colored precipitate or fluorescence. By deleting pieces of the regulatory DNA, the enhancers/silencers that regulate the expression of the gene can be studied. A reporter construct is used to do
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3-2-09_gene_expression - Zoology 470 2009 Course handouts...

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