Study_Guide_for_Chapter_21 - BMB 402H STUDY GUIDE Spring...

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BMB 402H STUDY GUIDE Spring 2009 Chapter 21: The TCA Cycle 1. The three stages of catabolism 2. Why the TCA cycle is the hub of metabolism 3. The three kinds of subunits of the pyruvate dehydrogenase complex, and the five coenzymes that participate in the reactions; be familiar with the five reactions and their mechanisms 4. For the coenzymes of the pyruvate dehydrogenase complex, you only have to know how they participate in the reactions; pay attention to the business end of TPP and lipoic acid, and there is no need to memorize their entire structures 5. How a single lipollysyl chain carries out all the three reactions in which it participates 6. Reasons why thioester bonds are high-energy bonds; the bond that is cleaved when a thioester participates in a nucleophilic displacement reaction 7. Chemical structures and names of all the intermediates in the TCA cycle; names of all the enzymes involved; pay attention to the carbons released in the oxidative decarboxylation steps; make sure that you can track the fates of the six carbons of glucose through one run
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