Study_Guide_for_Chapter_17 - BMB 402H STUDY GUIDE Spring...

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BMB 402H STUDY GUIDE Spring 2009 Chapter 17 Glycolysis 1. Names and chemical structures of all the intermediates of glycolysis, and names of the enzymes involved in each step of the pathway 2. Chemical strategy of glycolysis 3. Be familiar with the mechanisms of all 10 glycolytic enzymes, as well as lactate dehydrogenase and pyruvate decarboxylase (yeast) (Note: You will NOT be asked to draw any mechanism on the exam, so you only have to know the mechanism well enough to understand the nature of each reaction.) 4. Why Mg 2+ is absolutely required for the activity of hexokinase and most other kinases 5. Two major differences between hexokinase and glucokinase that were discussed in class 6. How glucokinase prevents wasteful hydrolysis of ATP 7. Fundamental difference between the mechanisms of class I and class II aldolases (also see pp. 589-591 of Voet & Voet) 8. For the aldolase reaction, make sure that you can follow the fates of carbons 1 through 6 of glucose
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