Shen_Study_Guide_withanswers

Shen_Study_Guide_withanswers - Study Guide Shen et al....

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Study Guide Shen et al. (2007) Selective activation of cognrate SNAREpins by Sec1/Munc18 proteins. Cell 128: 183-195. These questions are intended to help you read the paper in an inquisitive fashion and to help you focus on some of the issues you should understand. Please cover each of these questions during your reading and studying. Please submit the answers to 5 of these questions for your homework assignment. 1. Based on the introduction, what were the major models for SM protein function before this paper? -SM proteins stimulate fusion -SM proteins block fusion by binding closed SNARE 2. How has the defined fusion system as used in Figure 1 been modified since the Weber paper (aside from the addition of Munc18-1)? -TIME of preincubation is much shorter -way data is shown (Rounds of fusion) is different -ratio of protein to lipid is lower (although different units are used for comparison so it is hard to know by how much) -the basics of the assay have actually changed very little though! 3. What was added to the negative control (no fusion at all) in Figure 1? Why does it
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Shen_Study_Guide_withanswers - Study Guide Shen et al....

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