Intro_Sect_1 - I. Overview of Section 1: Organelle...

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Class 3: Frye, L. D. and M. Edidin (1970) The rapid intermixing of cell-surface antigens after formation of mouse-human heterokaryons. J. Cell Science 7:319-335. This classic paper is an elegant demonstration of the ±uid mosaic model of membrane structure. Class 4: Introduce nuclear transport and SNARE hypothesis to set up papers 2-4. Class 5: Kutay et al. (1997) Export of importin α from the nucleus is mediated by a speci²c nuclear transport factor. Cell 86:1061-1071. These authors de²ne an “exportin” that helps complete the asymmetric cycle of nuclear protein import and export. Class 6: Experimental approaches exercise 1 / QUIZ #1 Class 7: Weber et al. (1997) SNAREpins: Minimal machinery for membrane fusion. Cell 92:759-772. These authors de²ne the minimal protein components of a membrane required to fuse two membranes. Class 8: Shen et al.: (2007) Selective activation of cognate SNAREpins by Sec1/Munc18 proteins. Cell 128: 183-195. These authors come a step closer to ²guring out how the minimal machinery identi²ed in the Weber paper acts in vivo. Class 9: Wrap up / Experimental approaches exercise 2 / QUIZ #2 I. Overview of Section 1: Organelle biosynthesis and protein traf²cking
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A. Eukaryotes - 5 to 100 microns in diameter (prokaryotes - 1 to 10 microns). B. Eukaryotic cells contain numerous membrane bound organelles. II. Introduction: Membranes (MBOC 12.1) .
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A. Eukaryotes - 5 to 100 microns in diameter (prokaryotes - 1 to 10 microns). B. Eukaryotic cells contain numerous membrane bound organelles. 1.Membranes provide reaction surfaces: •Electrical potential across plasma membrane which is important for signaling can be distinct from the electrical potential across mitochondrial membrane which is important for ATP synthesis. II. Introduction: Membranes (MBOC 12.1) . 2.Lumens provide unique reaction centers. •Lysozymes are full of degradative enzymes. •Nucleus allows •uncoupling of transcription and translation. •RNA splicing to precede translation.
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1. Fluid mosaic model - (MBOC 10-1) A. Cell membrane structure: •Bilayer is about 5 nm thick (this includes the hydrophobic core and polar head groups). •Each layer of a membrane bilayer is often called a lea±et.
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1. Fluid mosaic model - (MBOC 10-1) A. Cell membrane structure: •What cannot cross the membrane ? •What can cross the membrane
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This note was uploaded on 10/19/2009 for the course B M B 460 taught by Professor Hanna-rose,w during the Spring '08 term at Pennsylvania State University, University Park.

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Intro_Sect_1 - I. Overview of Section 1: Organelle...

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