{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}

Kutay lec notes - K u ta y e t a l 1 9 9 7 E x p o r t o f...

Info icon This preview shows pages 1–8. Sign up to view the full content.

View Full Document Right Arrow Icon
Kutay et al. 1997 Export of importin α from the nucleus is mediated by a specific nuclear transport factor. Cell 86:1061-1071.
Image of page 1

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Kutay et al. 1997 Export of importin α from the nucleus is mediated by a specific nuclear transport factor. Cell 86:1061-1071. A. Major question •There is an assymetric import/re-export cycle: The import of cargo and the re- export of the receptors has to be coupled for import to be efficient. •How is importin α efficiently recycled to the cytoplasm for further rounds of import? •Importin α is part of the NLS receptor that mediates nuclear import of cargo proteins with a classic NLS. •Importin α− Importin β receptor binds NLS-containing cargo and mediates translocation across the nuclear pore •The complex is disrupted after importin β binds RanGTP in the nucleus. •Importin β acts as an export receptor when bound to RanGTP, but how is importin α exported? B. Hypothesis : •There must be an export receptor for importin α just like there is an import receptor for proteins that must enter the nucleus •an importin- α export receptor would have certain properties •Bind importin α together with RanGTP (nucleus) •release importin α when RanGTP is converted to RanGDP (in cytoplasm)
Image of page 2
*note:NTF2 is the import receptor for Ran Set up a system where proteins are transported across a nuclear membrane (a HeLa cell) C. Approach: Cell Biology and Biochemistry Experimental Strategy:
Image of page 3

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
C. Approach - permeabilization. System must be able to be manipulated •Cells permeabilized Is cytoplasmic envelope intact? Is nuclear envelope intact?
Image of page 4
C. Approach - permeabilization. System must be able to be manipulated •Cells permeabilized
Image of page 5

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
C. Approach - permeabilization. System must be able to be manipulated •Cells permeabilized •Soluble factors are lost •Allows authors to add defined components to the system while using the native nuclear envelope for transport
Image of page 6
C. Reconstitutionon •Add back energy (GTP and way to regenerate it from ATP - more phosphate available from creatine phosphate) AND
Image of page 7

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Image of page 8
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern