Unformatted text preview: the protein, and a secondary antibody that binds the first one, and also has an acitivity that allows it to be “seen” 3. Make sure you understand on what basis proteins are separated on SDS‐ PAGE gels‐ where do smaller and larger proteins run?, and generally how antibodies work. Look back and remind yourself of the difference between a monoclonal and polyclonal antibody. Which is likely to have more binding sites in any particular protein? A monoclonal will only recognize one epitope in a protein, but in a polyclonal there could be many different antibodies that recognize different epitopes....
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- Spring '08
- gel filtration column, beads. Large proteins