Class_3_highlights Frye and Edidin - Frye and Edidin...

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Frye and Edidin highlights Describe the general outline of the experimental strategy used to demonstrate the fluid membrane. use of two different cell lines method to fuse the cells - what does sendai virus do? be able to picture how this works time course of the experiment (fusion, incubation and then antibody staining) how are membrane proteins visualized? indirect immunofluorescence incubate with primary antibody that recognizes cell membrane protein incubate with secondary antibody that recognizes the prmary antibody note: secondary antibody is the one that is labeled with fluorescent tag visualize with fluorescece microscopy What is a cell line? What strategies can be used to make one? what happens to the cells? Understand the general strategy for creating the antibodies Why use two different types of animals (rabbit and mouse) for primary antibody production and only one type of animal (goat) for secondary antibody production? How were the antibodies tested to make sure they were specific for human or mouse
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This note was uploaded on 10/19/2009 for the course B M B 460 taught by Professor Hanna-rose,w during the Spring '08 term at Pennsylvania State University, University Park.

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Class_3_highlights Frye and Edidin - Frye and Edidin...

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