Class_4_highlights

Class_4_highlights - Intro Nuclear trafficking and vesicle...

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Intro : Nuclear trafficking and vesicle trafficking The nucleus is surrounded by a double membrane perforated by nuclear pores through which all protein traffic between the nucleus and the cytoplasm occurs. The lumen is continuous with the ER lumen. What proteins are capable of crossing the nuclear membranes? (only proteins with the appropriate nuclear localization signal or a small protein, less than 60kD) importin alpha and beta form the heterodimeric nuclear import receptor for proteins with a classic NLS. what does a classical NLS look like? mutation of a NLS can demonstrate that the NLS is necessary for nuclear localization transfer of a NLS to a gold bead is an experiment that can demonstrate that a NLS is sufficient for nuclear localization. which component of the dimeric (alpha/beta) receptor binds the NLS? which component of the dimeric receptor binds the nucleoporin protein in the nuclear pore? Remember that binding to the nucleoporin is what is required to get the complex across the nuclear envelope. KNOW HOW a small GTP binding protein (aka small G-protein) cycles between an
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Class_4_highlights - Intro Nuclear trafficking and vesicle...

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