Margolis_answers - Guide for Margolis et al(1989 EGF...

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Guide for Margolis et al. (1989) EGF induces tyrosine phosphorylation of phospholipase C-II: A potential mechanism for EGF receptor signaling. Cell 57:1101-1107. These questions are intended to help you read the paper in an inquisitive fashion and to help you focus on some of the issues you should understand. Please cover each of these questions during your reading and studying. Please submit the answers to 5 of these questions for your homework assignment. 1) Cell lysates were prepared to examine how EGF affects PLC-II. Orthovanadate, Na Fluoride, pyrophosphate and p-nitrophenyl phosphate were present in the lysis buffer. These inhibit phosphatase activities that are present in the lysate. Why was it important to inhibit dephosphorylation of proteins in the lysate? Phosphorylation is an important step in the signal transduction pathway. The authors want to know whether PLC-II is phosphorylated. To accomplish this, they must preserve the status of phosphorylation after lysing the cells by preventing anything that
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This note was uploaded on 10/19/2009 for the course B M B 460 taught by Professor Hanna-rose,w during the Spring '08 term at Penn State.

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Margolis_answers - Guide for Margolis et al(1989 EGF...

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