Olivier_et_al_guide_answers - Guide for Olivier et al....

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Guide for Olivier et al. (1993) A Drosophila SH2-SH3 adaptor protein implicated in coupling the sevenless tyrosine kinase to an activator of Ras Guanine Nucleotide Exchange, Sos. Cell 73: 179-191. These questions are intended to help you read the paper in an inquisitive fashion and to help you focus on some of the issues you should understand. Please cover each of these questions during your reading and studying. 1) The paper focuses on delineating a signal transduction pathway that goes between the receptor tyrosine kinase and the GTP binding protein called Ras. This type of signal transduction pathway plays a major role in the development of the Drosophila eye. We already looked at another pathway where a receptor tyrosine kinase, EGFR, phosphorylates phospholipase C gamma which in turn leads to the production of the second messengers IP3 and diacylglycerol. Many of the proteins that interact with active receptor tyrosine kinases, including PLCgamma, contain an SH2 domain. SH2 domains interact with regions of the receptor containing phosphotyrosine. Remember that the receptor becomes phosphorylated on tyrosines due to autophosphorylation. (SH2 stands for src homology 2 domain. Src is a cytoplasmic tyrosine kinase that is involved in regulating proliferation.) To study the signal transduction pathway, the investigators begin by screening for proteins that contain an SH2 domain. What was the probe that was used for this screen? 1) The probe was a portion of the EGF-R that had been phosphorylated on tyrosines with radioactive 32P. 2) Screening of the expression library identified a gene called drk (downstream of receptor kinase).
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This note was uploaded on 10/19/2009 for the course B M B 460 taught by Professor Hanna-rose,w during the Spring '08 term at Pennsylvania State University, University Park.

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Olivier_et_al_guide_answers - Guide for Olivier et al....

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