Highlights from class 12- Margolis paper

Highlights from class 12- Margolis paper - Lecture 12...

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Lecture 12 Highlights What is an RTK? EGF? EGFR? Describe the “pathway” from EGF to growth response in the cell. How does EGF "activate" the receptor? What role does dimerization play? What is meant by auto-crossphosphorylation? What residues become phosphorylated? What are some of the effectors that are activated by EGFR? How are they "linked" to the activated receptor? What is an SH2 domain and what does it bind to? -(we didn't get to the SH2 domain in today's lecture but I left it on here as a question because we will in the next lecture) Describe the "pathway" from PLC activation to activation of ca/calmodulin dependent kinase in the cell. What does PLC cleave? What are the products and what are their functions? Which product is soluble? Which is membrane bound? Understand how Co-IPs are performed in the Margolis paper including why phosphatase inhibitors are necessary, what is the role of triton X-100, how are the immuno-protein complexes “pulled” out of the solution and how proteins are visualized on the blot of the
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This note was uploaded on 10/19/2009 for the course B M B 460 taught by Professor Hanna-rose,w during the Spring '08 term at Pennsylvania State University, University Park.

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Highlights from class 12- Margolis paper - Lecture 12...

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