Guide for Nikolaev et al. (2003) Parc: A cytoplasmic anchor for p53. Cell 112:29-40. . These questions are intended to help you read the paper in an inquisitive fashion and to help you focus on some of the issues you should understand. Please cover each of these questions during your reading and studying. Please submit the answers to 5 of these questions for your homework assignment. 1. p53 is a transcription factor that is maintained in a latent form in most cells and is activated in response to certain stresses. Name one downstream transcriptional target of p53 that you have already learned about. What is the role of this target protein? 2. p53 is a transcription factor with a nuclear localization signal that obviously functions in the nucleus. Why are the authors looking for cytoplasmic proteins that might bind to p53? In other words, what is the primary hypothesis of this paper? 3. How are p53-binding proteins found and identified in Figure 1? 4.
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