Intro_cell_cycle

Intro_cell_cycle - 1 I. Proliferation - overview of a cycle...

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Unformatted text preview: 1 I. Proliferation - overview of a cycle Proliferation of "stem cells" replaces old cells. Proliferation heals wounds. But uncontrolled proliferation is a hallmark of many cancers. 1/6 of individuals in the developed world die of cancer. II. Cell cycle "check points III. Factors regulating progression through the cell cycle IV. Illustration of cell cycle control Cell cycle overview - Chapter 17 in MBoC 2 IA. Proliferation and differentiation are opposing pathways. Cell cycle coordinates events required for proliferation: DNA replication and cell division. Based on visible features, cell cycle is divided into 2 phases: 1. Interphase. Chromosomes not visible. 2. M phase / metaphase. Chromosomes in mammalian cells condensed / visible. Based on molecular analysis , 4 phases : 1. G 1 (Interphase) 2. S (Interphase) 3. G 2 (Interphase) 4. M phase (Metaphase) lack of growth signal in vivo (or in culture) or terminal differentiation is accompanied by withdrawal to G phase 3 IB. Proliferation involves execution of the cell cycle. 1. G 1 phase - decision to go through cell cycle (in response to growth signals) or become quiescent (enter G ) 2n DNA content DNA content can be monitored with FACS (Fluorescence Activated Cell Sorter). 9 hours in rapidly dividing cells. (MBOCed4 17-12) 4 IB. Proliferation involves execution of the cell cycle. 3. G 2 phase 4n DNA content 4.5 hours in rapidly dividing cells 4. Mitosis chromosome condensation nuclear breakdown spindle formation 0.5 hours in rapidly dividing cells. 2. S phase - DNA replication (MBOCed4 17-11) monitored by 3 H -thymidine uptake or BrdU uptake 10 hours in rapidly dividing cells 5 C. Yeast. Notable distinctions from mammalian cells. 2 hr. doubling time. Exhibits either a haploid or diploid life cycle: G 1 can be either 1n or 2n, respectively....
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Intro_cell_cycle - 1 I. Proliferation - overview of a cycle...

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