Lee lecture notes - 1 cdc genes Background A. Many...

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Unformatted text preview: 1 cdc genes Background A. Many cell-division-cycle (cdc) control genes were identifed in screens For yeast mutants with abnormal cell cycles (MBOC 17-4) . OFten, the screens are designed to identiFy conditional, temperature sensitive (ts) mutants since cell cycle deFects are expected to be lethal. Permissive temperature - proliFerate. Restrictive temperature - no proliFeration. 2 cdc genes Background A. Many cell-division-cycle (cdc) control genes were identifed in screens For yeast mutants with abnormal cell cycles The haploid liFe-cycle makes characterization oF the mutant phenotype relatively simple No problem characterizing a "recessive" mutation - no homologous chromosome carrying a wild-type copy oF the gene to mask the mutant gene. 3 cdc2 is a cyclin dependent kinase (cdk) Background B. The cdc2 mutant of S. pombe results in a cell-cycle arrest phenotype. At the restrictive temperature, a cdc2 ts allele causes cells to arrest in G2 with an elongated phenotype (they cant go through mitosis). Some dominant alleles, which appear to be "gain of function" mutations, drive the cells into mitosis before the cells have reached the the appropriate size. cdc2 has protein kinase activity. Its level is reduced in nutrient starved cells (cell cycle stops) but reappears when nutrients are added (cell cycle resumes). A similar gene is found in S. cerevisiae - CDC28. 4 Lee and Nurse (1987) Complementation used to clone a human homologue of the Fssion yeast cell cycle control gene cdc2. Nature 327: 31-35....
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This note was uploaded on 10/19/2009 for the course B M B 460 taught by Professor Hanna-rose,w during the Spring '08 term at Pennsylvania State University, University Park.

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Lee lecture notes - 1 cdc genes Background A. Many...

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