Baldin guide - Guide for Baldin et al. (1993) Cyclin D1 is...

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Guide for Baldin et al. (1993) Cyclin D1 is a nuclear protein required for cell cycle These questions are intended to help you read the paper in an inquisitive fashion and to help you focus on some of the issues you should understand. Please cover each of these questions during your reading and studying. Please submit the answers to 5 of these questions for your homework assignment. 1) To investigate the role of cyclin D1 in vivo, the authors obtain a cyclin D1 cDNA clone. They first use this clone to overexpress and purify cyclin D1 from bacterial cells (this is called recombinant cyclin D1) and produce an anti-recombinant cyclin D1 antibody. Second, they immunoprecipitate (IP) protein from human cells using this antibody. Figure 1 presents their data demonstrating that the protein they IP from human cells is indeed cyclin D1. How does the experiment in Figure 1A, lane 6 (there is no protein in the IP in lane 6) demonstrate the specificity of their antibody for cyclin D1? 2) The authors have also produced cyclin D1 protein by transcribing and translating their
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This note was uploaded on 10/19/2009 for the course B M B 460 taught by Professor Hanna-rose,w during the Spring '08 term at Penn State.

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Baldin guide - Guide for Baldin et al. (1993) Cyclin D1 is...

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