Highlights from Dulic paper

Highlights from Dulic paper - Lecture 20 Dulic et al...

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Lecture 20 Dulic et al Highlights Understand the various levels of control / levels of regulation on cdk activity that we have discussed. cyclin association phosphorylation (inhibitory and stimulatory) and dephosphorylation (of inhibitory phosphorylation) what is the role of CAK? of wee1? of cdc25? cdk inhibitors How do Dulic et al synchronize cells? How do they monitor S phase progression? Can you name 1 or 2 other ways to synchronize cells (e.g., the previous paper used another example)? What about yeast cells – can you synchronize them? What happens to cyclinE ACTIVITY in response to DNA damage? How is this measured? What causes this effect? Since cyclin E levels are unaffected by DNA damage, the authors investigate 3 other alternative hypotheses for why cyclinE activity is inhibited. What are these hypotheses/ which steps in the pathway are they looking at? How are they tested? Which one turns up a positive result? How is Figure 1, panel C (looking at Rb) evidence that cyclin D and cyclin E ACTIVITY
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Highlights from Dulic paper - Lecture 20 Dulic et al...

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