Highlights from intro to cell cycle - Highlights - Section...

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Highlights - Section 3 - Lecture 17 Name the cell cycle stages and the major events of each stage. o How can you measure the percentage of cells in a population that are in G1? in G2? in mitosis? o How do you use BrdU and 3H-thymidine to measure the percentage of cells in a population that are in S phase? o Take a look at the FACS-derived graph and interpret whether most cells are in G1, G2 or S. How does this relate to the length of each phase? o What is G 0 ? Why would / what kind of a cell would be in G0? Do you understand how FACS works? o You have to have very dilute cells and send them through the detector one at a time – they can still be alive o You must have a detectable fluorescent label – for example, fluorescent dye bound to the DNA (can still be alive) o The instrument can detect the amount of fluorescence and plot a graph of number of cells with “low”, “medium” or “high” fluorescence o When using a DNA dye, “low” tells you how many cells are in G1,
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This note was uploaded on 10/19/2009 for the course B M B 460 taught by Professor Hanna-rose,w during the Spring '08 term at Pennsylvania State University, University Park.

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Highlights from intro to cell cycle - Highlights - Section...

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