Highlights from Lee paper - Lecture 18 Highlights Be able...

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Lecture 18 Highlights Be able to discuss the advantages of using yeast for cell cycle studies advantage of haploid life cycle? advantage/power of temperature sensitive mutations? Can you explain one way a mutation can cause a ts phenotype? advantage of the cellular morphology? What is the biological activity of cdc2 in S. pombe? What type of enzymatic activity does cdc2 have? What happens to cdc2 temperature sensitive mutant yeast at the restrictive temperature? Morphologically? Molecularly? (e.g., which stage of cell cycle are they stuck at? ) What kinds of tests could you do to prove they are stuck at this stage? What happens to gain-of-function cdc2 mutants? Understand how to apply a complementation strategy to screen a human cDNA library for a functional homolog of a yeast protein. What was the function of the leu plasmid in the original screen? What is the function of the SV40 promoter + cDNA plasmid. What organism was this originally designed to express in? What is the function of the “Amp” and “ori” sequences on the cDNA plasmid?
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This note was uploaded on 10/19/2009 for the course B M B 460 taught by Professor Hanna-rose,w during the Spring '08 term at Pennsylvania State University, University Park.

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Highlights from Lee paper - Lecture 18 Highlights Be able...

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