Highlights from Nikolaev paper

Highlights from Nikolaev paper - Highlights - Section 3 -...

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Highlights - Section 3 - Lecture 21 - Nikolaev In addition to halting the cell cycle for repair, what other important (and longer term) response do mammalian cells have to severe DNA damage (damage that can’t be repaired)? What is a proto-oncogene? give examples What is an oncogene? What is a tumor suppressor? give examples Explain why p53 mutations act as dominant negatives whereas mutations in tumor suppressors are generally recessive. Describe the role of p53 in responding to DNA damage or other stress. Name two ways to down-regulation of p53 under non-stressed conditions. Proteolysis Sequestering in the cytoplasm Describe the general strategy that was used to identify Parc. What is a FLAG tag and how was it used? Why did the authors overexpress a mutant version of p53 (the 175 Arg to His) How was the parental cell line that had no p53 useful? ( I can think of two ways. ...1. it was a good control to make sure the identified proteins were actually specific in the actual affinity purification experiment. 2) It was necessary as the parental cell line to
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Highlights from Nikolaev paper - Highlights - Section 3 -...

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